Nitrification Performance and Microbial Community Analysis in Carbon Membrane-Aerated Biofilm Reactor
炭膜曝气生物膜反应器硝化作用及其微生物群落结构分析

A carbon membrane-aerated biofilm reactor was developed to treat nitrogenous inorganic wastewater. Influent NH; -N concentrations and HRT were changed to investigate nitrification performance of reactor,oxygen utilization and NH4+ -N's removal loading. Biofilm's surface characteristics and dominant bacteria of nitrifier were analyzed. The results show that under the conditions of intra-membrane pressure of 0.017 MPa, influent NH4+ -N of 50 mg/L and HRT of 8 h NH4+ -N removal efficiency reaches 96% and effluent average nitrite is 17 mg/L, which benefits short-cut nitrification to a certain extent. The bacteria within biofilm consume all oxygen supplied through carbon membrane. The maximum specific removal rate of NH4+ -N is 9.7 g/(m2 x d), which is limited by the amount of bacteria grown onto carbon membrane's surface. Fluorescent in situ hybridization analysis indicates that within the biofilm Nitrosomonas and Nitrosospira are main ammonia-oxidizing bacteria and occupy about 19% and 21% of the total bacteria number, respectively. The Nitrobacter are not observed and Nitrospira are dominant nitrite-oxidizing bacteria, the fraction of which is 20% of total bacteria.