Copper Speciation in the Gill Microenvironment of Cyprinus carpio
铜在鲤鱼(Cyprinus carpio)鱼鳃微环境中的形态分布

Concentrations of free copper and mucus and other parameters including pH and alkalinity of the gill microenvironment of Cyprinus carpio exposed to copper of various levels were determined using Playle's apparatus.MINEQA2 was applied to calculate the copper speciation and singlesite complexation model was employed for characterizing the complexation capacity of gill mucus for copper.A procedure of trial and error with the two approaches involved was developed for the speciation calculation.It was shown that excretion of mucus was positively correlated to the total copper level in water.It was found that in the gill microenvironment,both pH and alkaline are higher than that in the bulk water,causing the difference in copper speciation.From 3 to 20% of copper in the microenvironment was mucus complexed.