Microbial Community Structure in Bio-Ceramics and Biological Activated Carbon Analyzed by PCR-SSCP Technique
生物陶粒与生物活性炭上微生物群落结构的PCR-SSCP技术解析

Analyses of microbial community structure in bio-ceramics(BC) and biological activated carbon(BAC),which widely used in drinking water treatment were performed by polymerase-chain-reaction-single-strand-conformation-polymorphism (PCR-SSCP) targeted eubacterial 16S ribosomal RNA gene.Microorganisms on bio-ceramics and biological activated carbon were detached by ultrasonic,culturing on R2A and LB agar,respectively,followed by genome DNA extracting.Results show that larger than 10 kb genome DNA could be extracted from all the samples except the BAC samples processed by ultrasonic.However,quantities of the extracted DNA were different.408 bp gene fragments were observed after PCR using the extracted genome DNA as templates.These gene fragments were digested with lambda exonuclease followed by SSCP electrophoresis.Same SSCP profiles were observed between ultrasonic eluting,R2A and LB agar culturing.The identity of the segment from bio-ceramics with uncultured Pseudomonas sp.Clone FTL201 16S rDNA(GenBank,AF509293.1)fragment was 92%,and identities of the two segments from BAC with Bacillus sp.JH19 16S rDNA(GenBank,DQ232748.1)fragment and Bacterium VA-S-11 16S rDNA(GenBank,AY395279.1)fragment were 100% and 99%,respectively.