Intracellular Storage Polymer Driven Simultaneous Nitrification and Denitrification of GAOs Granular Sludge
聚糖菌颗粒污泥基于胞内储存物质的同步硝化反硝化

Glycogen accumulating organisms(GAOs) granular sludge was cultivated and stably existed in a sequencing batch reactor(SBR),fed with acetate as organic substrate,by means of special alternating anaerobic-aerobic operation mode, i.e.withdrawal after anaerobic period and sludge aeration during aerobic period to suppress the activity of phosphate accumulating organisms(PAOs).Anaerobicaerobic batch tests of GAOs granular sludge were carried out to investigate its simultaneous nitrification and denitrification(SND) performances under different TOC/N.The results showed GAOs granular sludge had high SND activity,SND efficiencies were 96.4%,95.3% and 96.2% respectively as TOC/N were 5.0,4.0 and 2.8,and the total nitrogen removal efficiencies,decreased with TOC/N decreased,were 66.0%,61.2% and 56.3% respectively.Measuring and analyzing the variation of ammonia,nitrite,nitrate,TOC,intracellular glycogen and PHB in batch tests,it was verified that intracellular PHB was used as carbon source of denitrification and denitrifying glycogen accumulating organisms(DGAOs) were responsible organisms for the denitrification activity.The degradation rate of PHB was slower than that of soluble substrate and therefore throughout the aerobic period the separate proceses of nitrification and denitrification can proceed at similar rates,which was help to improve SND efficiency.