Effect of Fe and Fe2+ on Hydrogen Production Capacity with Mixed Culture
Fe和Fe2+对混合细菌产氢发酵的影响

The effects of Fe and Fe2+ concentration in the external environment on hydrogen production capacity of ethanol type fermentative microorganism flora were investigated. The optimum range of Fe and Fe2+ concentration enhanced hydrogen production were obtained and the characteristic of fermentation types at different concentration of Fe and Fe2+ were discussed. It was demonstrated that both Fe and Fe2+ were able to enhance the hydrogen production capacity of microorganism flora. The maximum hydrogen production yield of 143.7mL·g-1 glucose and the maximum specific hydrogen production rate 21.2mL·(h·g)-1 were obtained at Fe2+ concentration of 200mg·L-1 and 50mg·L-1 . The maximum hydrogen production yield of 156 1mL·g-1 glucose and the maximum specific hydrogen production rate 23 5mL·(h·g)-1 were obtained at Fe concentration of 1000mg·L-1 and 500mg·L-1 . At the same concentration, the enhancement of Fe is better than Fe2+ when the concentration is higher than 50 mg·L-1. A sequential extraction technique was used to represent arbitrary divisions between different metal forms of Fe2+ in the mixed culture.