Endurance and Mechanisms of Soil Urease and Dehydrogenase Exposed to Reactive X-3B Red Dye Contamination
土壤脲酶和脱氢酶对活性X-3B红污染暴露的耐受性及机理研究

Endurance and mechanisms of urease and dehydrogenase in three Chinese typical soils (burozom, cinnamon soil and red soil) exposed to the contamination of reactive X-3B red dye were experimentally studied using the microcosmic method. The results indicated that the activities of urease and dehydrogenase were both promoted in the three soils by reactive X-3B red dye in low dose, 500 and 1000 mg/L although the stimulating intensity was different from each other. For one thing, the activity of urease in burozom was promoted mostly followed by that in red soil and that in cinnamon was promoted most weakly. For the other, reactive X-3B red dye in the same concentration could increase the activity of dehydrogenase in cinnamon soil most greatly and in burozom most weakly. However, the stimulating intensity of urease and dehydrogenase activities in the three soils was more and more fainter with the extension of days. Furthermore, reactive X-3B red dye in high concentration (3000, 5000 and 10000 mg/L) could not inhibit the activities of urease and dehydrogenase in tested soils. On the contrary, all of them remained the level of the activities in unpolluted soils and no toxicity of reactive X-3B red dye could reveal with time. Therefore, both enzymes in three types of soils had the tolerance to reactive X-3B red dye. Based on the experimental data, relevant endurant mechanisms were also analyzed and discussed.