Relationship of Heterogeneous Sensitivity to Metal Ion and Its Cell Cycle in Scenedesmus obliquus
栅藻对金属离子的异质敏感性与其细胞周期的关系

It is important to investigate the effect of contamination on the heterogeneous cells characteristically observed within single algae species,which can be helpful to understand the sensitivity of organism and the toxicity mechanism.Flow cytometry was used to assess sensitivity to metal ion(Cd2+ and Ni2+) in Scenedesmus obliquus.By determination of esterase activity in cell fractions gated by FSC(an indicator of the cell volume),it was shown that the resistance to metal ion was improved as the cell volume increased.By simultaneously examining FSC and DNA content,a relationship between the cell volume and the cell cycle stage was observed: as the cell volume increased,the S.obliquus cells changed from G1 phase to G2/M phase.The cells in G2/M phase had more resistance to metal ion.Thus,this observation indicate that the difference of cell cycle may be the important cause of heterogeneous sensitivity to metal ion in S.obliquus.