Study on the Microbial Film Immobilization Methods for comparing the Biosensors for Rapid BOD Determination
BOD快速测定传感器的微生物膜固定方法研究

Several physical and chemical methods, such as the sokium alginate-calcium chloride embedding process, the cellulose acetate sandwiching process and the bovine serum albumen-glutaraldehyde crosslinking process, were used to prepare the immobilized microbial films which were then combined with an oxygen electrode to make the newly developed biosensors for rapid BOD determination. The tests were carried out for the effects of different films on the performances of electrode, including its response sensitivity, linearity ranges, resPOnse time, stability and life-time. The performances of the biosensors which were formed under different process conditions such as PH values, temperatures, cronlinking agentS and the amounts of embedding agentS used were also compared. The results show that the biosensors prepared with the cross-linking process have the most preferred Performances, including an electrode life-time of up to 40 days, a BOD determination time of 10 to 15 minutes, and the reproducibility and accuracy of determinations both in compliance with the requirements of standard BOD determination.