Alterations of Oxidative Metabolism Respond to Cadmium Stress in Cd-Sensitive Mutant Rice Seedlings
镉敏感水稻突变体在镉胁迫下活性氧代谢的变化

Cd-sensitive mutant rice(Oryza sativa L.subsp.Japonica,cv.Zhonghua 11) was obtaired using our Agrobacterium tumefaciens based gene derived system.Significant phenotypic differences between a Cd-sensitive mutant(ST) and wild type(WT) rice seedlings by Cd2+ treatment was observed.Results indicated the mutant had more intensive capability of cadmium transport in leaf,which leaded to more level of lipid peroxidation than that of the wild type.Furthermore,A rapid Cd-induced O·-2 and H2O2 increase in the mutant leaves was observed,which induced activity of antioxidant enzymes.The maintaining of SOD activity for resisting stresses in leaves of the mutant was limited in vivo.The CAT activities in leaves of the mutant were more inhibited than that of wild type,which might be a physiological explanation for sensitivity to cadmium in the mutant.However,the difference of the G-POD activities in leaves between the mutant and wild type was not significant.