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环境科学  2006 

Production of Ligninolytic Enzymes in Bioreactor
白腐真菌木质素降解酶的反应器发酵

Keywords: white rot fungus,P,ligninolytic enzyme,fed-batch,bioreactor
白腐真菌
,黄孢原毛平革菌,木质素降解酶,分批补料,生物反应器

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Abstract:

Production of ligninolytic enzymes under nitrogen limited conditions(C/N=56/2.2) was studied in a 5-L stirred tank bioreactor with a working volume of 2 L for obtaining higher production of ligninolytic enzymes by white rot fungus Phanerochaete chrysosporium BKM-F-1767 and its control strategy.Results show that the manganese peroxidase(MnP) and laccase(Lac) reached peak at the sixth day and the seventh day,respectively,and the variation of them with time in a batch cultivation are similar to the results by agitated Erlenmeyer flasks;however higher enzyme activity was not achieved by applying a fed-batch strategy,in which nitrogen limited medium was fed to the reactor.In addition,variation of pH during cultivation was related to the growth of P.chrysosporium and enzymes production during both batch and fed-batch cultivation.The pH value of liquid medium began to decline when the enzyme activity occurred in the system,and the decline became more and more slow along with the decrease of enzyme activity at the end of fermentation.So,pH would be as a control parameter to find out the growth of P.chrysosporium and enzymes production during incubating P.chrysosporium.However,fed-batch strategy still need further study.

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