Isolation of an Amylolytic Lactic Acid Bacterium and Its Application on Lactic Acid Production from Kitchen Waste
解淀粉乳酸细菌在厨余垃圾乳酸发酵中的应用

In order to realize resource recycling of kitchen waste, the enhancement of fermentative production of lactic acid (LA) by a strain of amylolytic lactic acid bacterium (ALAB) isolated from kitchen waste was investigated. A total of 6 strains of ALAB were isolated from anaerobically fermented kitchen waste. Among these isolates, the strain FH164 exhibited the highest starch-hydrolyzing rate and LA production. 32.67 g x L(-1) of LA was produced from 40.50 g x L(-1) of soluble starch by strain FH164 at pH 5.5-6.0 in 48 h of fermentation. On the basis of its morphological and physio-biochemical characteristics as well as 16S rDNA sequence, the strain FH164 was tentatively identified as Streptococcus sp.. 28.23 g x L(-1) of LA was obtained from kitchen waste by the strain FHl64 employing an open fermentation mode (substrate non-autoclaved), which was 19.2% higher than that of the fermentation without inoculum (control).