2-DE ANALYSIS OF LIVER OF PSEUDOSCIAENA CROCEA AFTER LOW TEMPERATURE BREEDING
低温选择大黄鱼(Pseudosciaena crocea)的肝脏蛋白质组双向电泳分析

We used 2-DE to compare the liver proteomes of Pseudosciaena crocea with or without low-temperature breeding, and found similar number of proteins expressed in the low-temperature breeding group (1673±47) and the control group (1651±43). The expressions of 19 proteins were significantly altered after low temperature breeding. Three proteins (MHC, CFL2 and 2-Cys Prxs) were identified by MALDI-TOF-MS/LC-MS-MS and MS-BLAST searching. The expressions of MHC and CFL2 protein were up-regulated remarkably, whereas the expression of 2-Cys Prxs was down-regulated remarkably, suggesting the anti-apoptosis ability of the low-temperature breeding group is strengthed.