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海洋与湖沼 2009
SEQUENCE COMPARISON AND SYSTEMATIC ANALYSIS OF mtDNA 16S rRNA GENOMIC FRAGMENTS OF LUTJANUS
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Abstract:
MtDNA 16S rRNA genomic fragments of nine Lutjanus were amplified by PCR;and the purified product yielded a systematic sequence with 561bp being sequenced.The contents of A,T,G and C are respectively 28.5%,22.1%,23.6% and 25.8%.The contents of AT are slightly higher than those of GC,and the difference in base composition in 18 Lutjanus species is the minimum.Comparison with MEGA 3.1 software revealed 72 variation loci,of which 44 are information loci.For base replacement,75.61% are caused by transition in it...