THE IMMUNITY OF ACANTHOPAGRUS SCHLEGELI INJECTED WITH OUTER MEMBRANE PROTEIN AND LIPPOLYSACCHRIDE OF ANTARCTIC ULTRAMICROBACTERIA ANT52 (ALTEROMONAS STELLIPOLARIS)
南极超微细菌ANT52(Alteromonas stellipolaris)外膜蛋白和脂多糖的提取物对黑鲷的免疫活性研究

The immunity competence of Acanthopagrus schlegeli was studied by injecting with crude outer membrane protein (OMP) and lippolysacchride (LPS) extracted from Antarctic ultramicrobacteria ANT52 (Alteromonas stellipolaris) in different combinations. Four parameters: the phagocytic activity of leucocytes in blood, and the activities of lysozme, antibacteria, and phenoloxidase in serum of immunized fish, being used to represent the non-specific immunogenicity, were determined in 1, 7, 14, 21, 28, and 35 days after the injection. The results showed that the four parameters rose significantly during the experimental period (P<0.05). These parameters of single OMP or LPS treatment group were greater than those injected with OMP+LPS combination treatment group; and reached peak value in 28 days post vaccination. It indicates that immunization with OMP and LPS of ANT52 could stimulate and enhance the function of non-specific immunity of A. schlegeli. Future studies are needed to understand the mechanism of the immunity competence.