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海洋与湖沼 2012
ISSR ANALYSIS ON GENETIC STRUCTURE OF FOUR MYTILUS CORUSCUS POPULATIONS
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Abstract:
The genetic structure of four geographic populations of Mytilus coruscus along the coast of China was investigated by the inter-simple sequence repeat (ISSR) fingerprinting of 120 individual clams. Of the 192 ISSR loci tested, 188 (97.92%) were polymorphic with 13 ISSR primers. The genetic diversity was high at species level. The result showed that the PPL about four populations were among 81.77%—89.58%, H were among 0.2950—0.2818, I were among 0.4213—0.4447, respectively for the four populations with the lowest values in FZ population, the highest values in WZ population. The GST of the four populations was 0.1519, Nm was 3.0576—5.9714, AMOVA analysis showed that the genetic variation mainly from individuals within populations. Genetic distance and UPGMA tree showed that WZ was clustered with ND into one clad, then with ZS, and finally with FZ. The result showed that the further geographical distance, the higher genetic distance; the genetic distance is proportional to the geographical distance.