PHENOTYPIC AND MOLECULAR CHARACTERIZATION OF PATHOGENIC VIBRIO PARAHAEMOLYTICUS ISOLATED FROM PENAEUS VANNAMEI
凡纳滨对虾(Litopenaeus vannamei)病原副溶血弧菌(Vibrio parahaemolyticus)的表型及分子特征

A mass mortality of cultured Litopenaeus vannamei broke out during summer in a local farm in northern Jiangsu Province.Syndromes include swimming near water surface and the pond walls,with black gill and opaque muscle.Tissue samples were taken in the liver,and strong pathogenicity to L.vannamei,P.japonicus and P.chinensis were determined by immersion.The phenotypic characteristics of isolates(4 strains) were examined in morphology,physiology and biochemistry;the 16S rRNA and gyrB were amplified by PCR and c...