THE SPECIFIC ALLERGENS IN CRAB SCYLLA SERRATA
锯缘青蟹(Scylla serrata)特异性过敏原的研究

To study the specific allergen in crab Scylla serrata,soluble protein from the muscle of S.serrata was extracted,and then separated by SDS-PAGE.With the positive and negative sera of anaphylactic patients,the special allergen was identified with the Western Blot,showing that the 14.3kDa was the specific allergen.The protein of 14.3kDa was then analyzed by gel filtration in ion exchange chromatography,and the MALDI-TOF/TOF-MS search on the Internet.The results indicate that the protein was hemocyanin subunit...