OBSERVATION ON THE DEVELOPMENT OF BLOOD CELLS OF CHELONIA MYDAS
绿海龟(Chelonia mydas)血细胞发育过程的观察

The blood cells of Chelonia mydas was studied through observation on stained imprint and smear of the peripheral blood, the bone marrow, the spleen and the liver etc. The stain method used is Wright's. The results show that the bone marrow and spleen were the main hemopoietic organs in C. mydas. Erythrocytes, monocyte and granulocytes developed mainly in the bone marrow; and lymphocytes developed mainly in the spleen. The spleen with late immature erythrocytes could be the place of maturation of erythrocytes. No primitive blood cells were observed in liver, which implies that the liver was not the hemopoietic organ in C. mydas. The development of erythrocytes includes 5 stages: primitive, early, middle, late, and mature. The body sizes of these cells increased first, then decreased, and increased in the end. The development of granulocytes includes 5 stages also: myeloblast, promyelocyte, meddle immature granulocytes, late immature granulocytes, and mature granulocytes. The body sizes of them decreased; and the sublobe of the nuclear may indicate the aging. All the four types of blood cells changed from with-nucleolus to without-nucleolus. Mitosis may take place in the primitive blood cells and immature ones, and direct division may occur in some mature red blood cells in the peripheral blood system.