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海洋与湖沼 2009
ANIMAL PROTEIN ENZYMATIC HYDROLYSIS, N, AND P SOURCES: COMBINATION EFFECT ON GROWTH AND BIOCHEMICAL PARAMETERS OF CHLORELLA VULGARIS
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Abstract:
The effects of different combinations of animal protein enzymatic hydrolysis and N (NH4HCO3), P (NaH2PO4) on growth and biochemical parameters of Chlorella vulgaris are studied. The optimal combinations were determined by the index of biomass, chlorophyll content and protein content by orthogonal design test. The results show that: (1) Interaction occurred among protein enzymatic hydrolysis, N, and P sources. The influencing factors on the biomass were P concentration and protein enzymatic hydrolysis, and those on the protein content of C. vulgaris was the combination of protein enzymatic hydrolysis and N concentration. (2) The optimal combination conditions (P<0.05) to increase the biomass, chlorophyll content and protein content of C. vulgaris are: N concentration at 50mg/L, P at 4mg/L, and protein enzymatic hydrolysis at 12ml/L.