GENETIC DIVERSITY OF DIFFERENT ECOLOGO-GEOGRAPHICAL POPULATIONS OF YELLOW CATFISH PELTEOBAGRUS EUPOGON
黄颡鱼(Pelteobagrus eupogon)不同生态地理分布群体遗传多样性的微卫星分析

Forty microsatellite loci were used for analyzing six populations of yellow catfish Pelteobagrus eupogon: YL the Yueliang (Moon) Lake, Jilin Province, 30 samples], SC (Sichuan Province, 30 samples), SH (Songhua River at Harbin, Heilongjiang Province, 18 samples), YY (the Changhu Lake, Jingzhou, Hubei Province, 30 samples), QQ (the parent generation of YY, from the Changhu Lake also), and TJ (Huanxin Breeding Farm, Tianjin, 30 samples). The observed (Ho), expected (He) heterozygosity, polymorphic information content (PIC), and number of effective alleles (Ae) were determined. Genetic similarity index and genetic distance were computed on the allele frequency. The Hardy-Weinberg Equilibrium was checked by x2 Test. Genetic differentiation (FST) and hierarchical partition of genetic diversity (Nm) were used. A dendrogram was built on the UPGMA results using PHYLIP software package (Ver.3.63). A total of 5042 fragments ranging from 150bp and 650bp in length were obtained. One to eight alleles were amplified in 40 loci and 143 alleles in all six populations. The average number of alleles in each locus is 3.575. The result shows that 1) the level of genetic variability is moderate in the six populations. The polymorphic information contents of the six populations are from 0.00 to 0.79, in average at 0.40, 0.37, 0.35, 0.39, 0.35 and 0.19, respectively. Effective alleles are from 1.00 to 5.44, in average of 2.05, 2.30, 1.93, 2.07, 1.93 and 1.47, respectively. The expected heterozygosity ranges from 0.00 to 0.83, in average at 0.45, 0.46, 0.36, 0.46, 0.41 and 0.23, respectively; 2) the highest genetic similarity index at 0.9947 was found between YY and QQ; and the lowest index at 0.4846 between SC and TJ. Correlation between the clustering result and the geographical distribution may exist.