GROWTH AND GENETIC DIVERSITY FOR FOUR SHELL COLOR LINES (F5) OF THE PEARL OYSTER PINCTADA MARTENSII
马氏珠母贝(Pinctada martensii)四种壳色选育系F5 的生长及遗传多样性分析

Survival rate and growth traits were measured among four shell color lines and control group of the pearl oyster Pinctada martensii. Growth traits contained shell length, shell height, shell width, hinge line, body weight, tissue weight and shell weight. The result showed that significant variation existed in these groups. Genetic diversity of four shell color lines of P. martensii was analyzed using microsatellite DNA. 30 pairs of primers were designed for this research. The result shown that there were 8 polymorphic loci and the proportion of polymorphic loci was 26.67%. Total 42 alleles were observed at the 8 loci from 120 individuals, with an average of 5.25 alleles per locus. Average expected and observed heterozygosities were 0.6850, 0.6831, 0.6730, 0.6622 and 0.3750, 0.2708, 0.4667, 0.3208 for the four lines, respectively, which showed that high level genetic diversity and breeding potential existed in the four lines. Average polymorphism information contents were 0.6190, 0.6182, 0.6130, 0.6025, 0.6131, and D values of heterozygote deviation were ?0.4526, ?0.6036, ?0.3065, ?0.5156 for the four populations, respectively. Genetic differentiation and genetic distance analysis showed that white shell color line and red shell color line were genetically close while black shell color line and white shell color line were distant.