Extraction of Industrial Lipase with CTAB-based Mixed Reverse Micelles
CTAB混合反胶团萃取工业脂肪酶

The extraction of industrial lipase with mixed reverse micelles formed by CTAB with Tween, TRPO, TBP and butyl acetate was investigated. The transfer of industrial lipase to the mixed reverse micelles formed from CTAB with Tween85, TRPO, TBP, butyl acetate was higher than that of the reverse micelles formed with CTAB reverse micelles. The addition of TRPO, TBP and butyl acetate to the CTAB reverse micelles had no effect on the backward transfer of industrial lipase. The mixed reverse micelles formed from CTAB with Tween60, Tween40 could obviously enhance the industrial lipase backward transfer. The activity recovery of industrial lipase with the mixed reverse micelles formed from CTAB with TRPO was about 70%, which was the best in these mixed reverse micelles tested.