Oxidation and Antioxidation of Hemoglobin in the Process of Membrane Separation
血红蛋白在膜分离过程中的氧化规律和抗氧化探索

The effect of different antioxidation agents on the reduction of methemmoglobin (MetHb) was investigated. Five antioxidation agents, i.e. vitamin C (Vc), reduced glutathione (GSH), cysteine, n-acetul-cyateine and sodium sulfite were used and compared according to different changes of MetHb content. The experimental results showed that all the antioxidation agents could protect the bioactivity of Hb from oxidation, while Vc had strong ability to reduce MetHb. The optimal conditions Vc/Hb=8 (mole ratio), pH=8] obtained were applied to the membrane filtration process following the ion-exchange chromatography step. Bioactivity of the hemoglobin was well maintained when Vc was added as antioxidation agent, and the content of MetHb was successfully controlled to 2.3% in the final purified hemoglobin product.