Fed-batch Cultivation of Botryococcus braunii in an Air-lift Photobioreactor
产烃葡萄藻在气升式光生物反应器中的分批补料培养

The culture of Botryococcus braunii was carried out in a 3 L air-lift photobioreactor. The batch cultivation results showed that the concentration of nitrate and phosphate ions decreased quickly by algal consumption, which limited the growth of Botryococcus braunii. Therefore, the fed-batch cultivation mode in which nitrate and phosphate were maintained in a certain range of concentration by multiple feeding in the late stage of fermentation was tested. Compared with batch cultivation, the cell concentration increased from 1.3 g/L to 1.9 g/L , hydrocarbon content increased from 22% to 29% on dry weight basis and the yield of hydrocarbon was greatly improved from 0.286 g/L to 0.551 g/L.