Application of Stoichiometric Displacement Model for Retention to the Chromatographic Separation by Molecular Imprinting Polymer
计量置换模型在分子印迹聚合物色谱分离中的应用

In the presence of template molecule of N-Boc-L-Tryptophan, molecular imprinting polymers (MIPs) were synthesized and evaluated by liquid chromatography. The results showed that the racemic N-Boc-Tryptophan was efficiently separated. Interaction of template molecule and stationary phase or mobile phase was analyzed with the stoichiometric displacement model for retention (SDM-R). It was showed that when absorption occurred between template molecule and MIPs, the molecule number of the displacing agent (isopropyl alcohol) released from the surface of the stationary phase was 1.2 fold that of its enantiomer, and the molecule number of the displacing agent released from a solute molecule was 1.5 fold that of its enantiomer. All these results indicated that the molecule recognition depended mainly on the structure between template molecule and MIPs.