Effects of He-Ne Laser Irradiation on the Mutagenesis of Beauveria bassiana
He–Ne激光对球孢白僵菌生物学特性的影响

The influences of He-Ne irradiation on the mutation and growth of Beauveria bassiana 75A strain was investigated. The optimum He-Ne laser irradiation dosage was 0.48 mW/cm2 for 15 min. Among the two ways of He-Ne laser irradiation, irradiation on bacteria suspension in physiological salt water appeared more effective in activation. Based on the analysis of the sporulation fermentation and biomass, it was found that He-Ne laser could give rise to a markedly biological stimulating effect and certain mutagenesis effect. Several strains that produced sporulation abnormally were screened. By the contrast of RNA and DNA contents from the extracell, intracell and periplasmic between mutant and original strains, further evidence that the He-Ne laser beam causes mutagenesis of Beauveria bassiana is concluded .