Effect of Shear Stress on Suspension Cultures of Taxus chinensis var. mairei
剪切力对南方红豆杉细胞悬浮培养的影响

Defined laminar shear field was created for the suspension cultures of Taxus chinensis var. mairei in a Couette-type shear reactor. It was found that the suspension cultures in the latter stage of exponential growth and early in the stationary phase were more susceptible to shear damage than cultures in the lag phase and early exponential phase, resulting in the most loss of the cell viability and the most accumulation of phenolics. Additionally, the shear stress caused the defense responses of Taxus chinensis var. mairei such as influx of H+, accumulation of phenolics and oxidative burst.