Light Transfer and Its Effect on Cell Growth in the Culture of Transgenic Synechococcus sp. PCC7942
转基因聚球藻7942培养中光传递及其对细胞生长的影响

The variation of light distribution and average irradiance in a photobioreactor during batch culture of transgenic Synechococcus sp. PCC7942 carrying thymosin a1 gene was modeled, and their effects on algal cell growth were analyzed. Light attenuation in the suspension culture of the transgenic Synechococcus sp. PCC7942 could be predicted more accurately with a hyperbolic model than with the Lambert-Beer model. With algal cell concentration increasing, the average irradiance in the photobioreactor and the specific light energy absorbance rate decreased continuously, and illumination zone decreased gradually after 1.5 d cultivation, resulting in the reduction of the cell specific growth rate; since light energy absorbed in the volume unit was constant after 1.5 d, the algal cell was in the linear growth phase; with dark zone exceeded 60% of the volume of the photobioreactor after 5 d cultivation, energy used for cell maintenance increased and the cell growth rate decreased. The thymosin a1 expression level of recombinant microalgal cell was 4.53 mg/L for 8 d cultivation.