Influence of External Electric Field on Distribution of Phenylalanine in an Aqueous Two-phase System
外电场对苯丙氨酸在双水相体系中分配的影响

In order to overcome the influences of electrode reactions and the long distance between two electrodes on two-phase electrophoresis, a mini-multi-channel aqueous two-phase electrophoresis (mini-MATPE) equipment was designed, and the separation of phenylalanine in this equipment was investigated with dextran-polyethylene glycol-water as a working system. The influences of time, current intensity, initial solute concentration on two-phase concentration ratio, recovery ratio, total mass flux, and electric current efficiency was discussed. Based on the study, a semi-empirical correlation for predicting the maximum mass flux is suggested.