Fermentation of Cellulosic Pyrolysate for Producing Ethanol
纤维素热解液发酵制乙醇

The cellulosic pyrolysate mainly containing 1,6-anhydro-b-D-glucopyranose (levoglucosan) could be hydrolyzed to glucose and further fermented to ethanol with microorganisms. Among the various hydrolysis treatments, the highest glucose yield (17.35%) was obtained by 0.2 mol/L H2SO4 at 121oC for 20 min. Such hydrolysate treated by neutralization with Ca(OH)2 and absorption with diatomite for detoxification was used for fermentation to ethanol by Candida krusei YZ-1. The yield was 0.42 g/g (ethanol/glucose), up to 84% of the theoretical yield. Zymomonas mobilis performed very poorly in fermenting all treated pyrolyate to ethanol.