Characteristics and Mechanism for Three-dimensional UV-fluorescence Spectroscopy of Plasma
三维紫外血浆荧光光谱学特性及机理分析

The plasma three-dimensional UV fluorescence excitation and emission spectra,the wavelength of 220~320 nm,are obtained by FLS920 Combined Fluorescence Lifetime & Steady State Spectrometer.There is a wide peak with 100 nm in the fluorescence emission spectra near 334 nm,from 300 nm to 400 nm.It is presented that the peak derivers from many fluorophores interaction,which is mostly Tryptophan and tyrosine in plasma protein.In addition,the other proteins and small molecules in the plasma made the fluorescence peak widen by emitting fluorescence and indirect energy transfer or interaction,which may modulate the spectral characteristic.