Resolution of Confocal Scanning Microscope for Second-harmonic Wave
二次谐波共焦成像的分辨率

The three-dimensional intensity pulse function of a confocal scanning microscope for second-harmonic wave is derived and compared with that of a two-photon fluorescent confocal scanning microscope.By use of Fourier transformation,the three-dimensional intensity pulse function is theoretically investigated in frequency domain where the transverse and axial cut-off spatial frequencies,together with the transmission bands are given.The results show the optical resolution for second-harmonic wave is better than that for twophoton fluorescence.Such conclusion is also proved from the uncertainty principle.