Determination of Biogenic Amines by Capillary Electrophoresis Coupling with Continuous Wave-based Multiphoton Excited Fluorescence Detection
毛细管电泳——多光子激发荧光检测分析生物胺

Multiphoton excited fluorescence (MPEF) detection coupling to capillary electrophoresis (CE) has been proposed to analyze biogenic amines,such as putrescine,cadaverine,histamine,spermine,spermidine and phenylethylamine.The detection limit was found to be nM-level,with linear range of over two orders of magnitude for quantitation.It showed that the constructed CE-MPEF method was well suited for complex systems like bio-sample,with advantages of high separation performance,high detection sensitivity and low cost.Further,MPEF exhibited a capability for improving separation resolution after comparing with conventional single photon laser induced fluorescence.