New Technology for DNA Analysis Based on Multiphoton Excited Fluorescence-capillary Electrophoresis
基于多光子激发荧光-毛细管电泳的DNA分析新技术

A novel system, capillary electrophoresis (CE) coupled with continuous wave-based multiphoton excited fluorescence (MPEF) detection, was demonstrated for biomedical analysis. A model sample containing various fluorescent dyestuffs was separated using CE and detected with this unique MPEF detector. It is shown that this CE-MPEF system exhibites various advantages such as high performance separation capability, low mass detectability and spectral excitability in a wide rang. And this novel system is employed successfully for DNA analysis, which indicated a promising future of CE-MPEF method in biomedical analysis.