This study was performed to investigate the association of prohibitin with renal interstitial fibrosis (RIF) lesion and to explore the association of all-trans retinoic acid (ATRA) treatment with prohibitin expression in RIF rats. Rats were divided into three groups: the sham operation group (SHO), the model group subjected to unilateral ureteral obstruction (UUO), and the model group treated with ATRA (GA). Renal tissues were collected at 14 and 28 days after surgery, and the relevant indicators were detected. In comparison with the SHO group, the RIF index in the UUO group was markedly elevated ( p < 0.01), and the RIF index in the GA group was alleviated compared with that in the UUO group ( p < 0.01). Compared with the SHO group, the expression of prohibitin (protein or mRNA) in the UUO group was significantly reduced (each p < 0.01). Prohibitin expression in the GA group was markedly increased when compared with that in the UUO ( p < 0.01). The expression of TGF-β1 (protein and mRNA), protein expressions of Col-IV, fibronectin, α-SMA and cleaved Caspase-3, ROS generation and cell apoptosis index in the UUO group were markedly higher than those in the SHO group (all p < 0.01), and their expressions in the GA group were markedly down-regulated compared to those in the UUO group (all p < 0.01, respectively). The protein expression of prohibitin was negatively correlated with the RIF index, protein expression of TGF-β1, Col-IV, fibronectin, α-SMA or cleaved Caspase-3, ROS generation and the cell apoptosis index (each p < 0.01). In conclusion, lower expression of prohibitin is associated with the RIF, and ATRA treatment is associated with increased prohibitin, which can prevent the progression of RIF.
References
[1]
Bielesz, B.; Sirin, Y.; Si, H.; Niranjan, T.; Gruenwald, A.; Ahn, S.; Kato, H.; Pullman, J.; Gessler, M.; Haase, V.H.; et al. Epithelial Notch signaling regulates interstitial fibrosis development in the kidneys of mice and humans. J. Clin. Invest 2010, 120, 4040–4054.
[2]
Kushiyama, T.; Oda, T.; Yamada, M.; Higashi, K.; Yamamoto, K.; Sakurai, Y.; Miura, S.; Kumagai, H. Alteration in the phenotype of macrophages in the repair of renal interstitial fibrosis in mice. Nephrology (Carlton) 2011, 16, 522–535.
[3]
Morisada, N.; Nomura, M.; Nishii, H.; Furuno, Y.; Sakanashi, M.; Sabanai, K.; Toyohira, Y.; Ueno, S.; Watanabe, S.; Tamura, M.; et al. Complete disruption of all nitric oxide synthase genes causes markedly accelerated renal lesion formation following unilateral ureteral obstruction in mice in vivo. J. Pharmacol. Sci 2010, 114, 379–389.
[4]
Correa-Costa, M.; Semedo, P.; Monteiro, A.P.; Silva, R.C.; Pereira, R.L.; Goncalves, G.M.; Marques, G.D.; Cenedeze, M.A.; Faleiros, A.C.; Keller, A.C.; et al. Induction of heme oxygenase-1 can halt and even reverse renal tubule-interstitial fibrosis. PLoS One 2010, 5, e14298.
[5]
Manucha, W. Biochemical-molecular markers in unilateral ureteral obstruction. Biocell 2007, 31, 1–12.
[6]
Manucha, W.; Carrizo, L.; Ruete, C.; Molina, H.; Valles, P. Angiotensin II type I antagonist on oxidative stress and heat shock protein 70 (HSP 70) expression in obstructive nephropathy. Cell Mol. Biol. (Noisy-le-Grand) 2005, 51, 547–555.
[7]
Puppin, C.; Passon, N.; Franzoni, A.; Russo, D.; Damante, G. Histone deacetylase inhibitors control the transcription and alternative splicing of prohibitin in thyroid tumor cells. Oncol. Rep 2011, 25, 393–397.
[8]
Zhu, B.; Zhai, J.; Zhu, H.; Kyprianou, N. Prohibitin regulates TGF-beta induced apoptosis as a downstream effector of Smad-dependent and -independent signaling. Prostate 2010, 70, 17–26.
[9]
Li, Q.F.; Liang, Y.; Shi, S.L.; Liu, Q.R.; Xu, D.H.; Jing, G.J.; Wang, S.Y.; Kong, H.Y. Localization of prohibitin in the nuclear matrix and alteration of its expression during differentiation of human neuroblastoma SK-N-SH cells induced by retinoic acid. Cell. Mol. Neurobiol 2011, 31, 203–211.
Zhou, T.B.; Qin, Y.H.; Ou, C.; Lei, F.Y.; Su, L.N.; Huang, W.F.; Zhao, Y.J. All-trans retinoic acid can regulate the expressions of gelatinases and apolipoprotein E in glomerulosclerosis rats. Vascul. Pharmacol 2011, 55, 169–177.
[12]
de Rosa, S.; Cirillo, P.; Paglia, A.; Sasso, L.; di Palma, V.; Chiariello, M. Reactive oxygen species and antioxidants in the pathophysiology of cardiovascular disease: Does the actual knowledge justify a clinical approach? Curr. Vasc. Pharmacol 2010, 8, 259–275.
[13]
Lee, H.P.; Casadesus, G.; Zhu, X.; Lee, H.G.; Perry, G.; Smith, M.A.; Gustaw-Rothenberg, K.; Lerner, A. All-trans retinoic acid as a novel therapeutic strategy for Alzheimer’s disease. Expert Rev. Neurother 2009, 9, 1615–1621.
[14]
Liu, X.; Lu, L.; Tao, B.B.; Zhou, A.L.; Zhu, Y.C. Amelioration of glomerulosclerosis with all-trans retinoic acid is linked to decreased plasminogen activator inhibitor-1 and alpha-smooth muscle actin. Acta Pharmacol. Sin 2011, 32, 70–78.
[15]
Ratnam, K.K.; Feng, X.; Chuang, P.Y.; Verma, V.; Lu, T.C.; Wang, J.; Jin, Y.; Farias, E.F.; Napoli, J.L.; Chen, N.; et al. Role of the retinoic acid receptor-alpha in HIV-associated nephropathy. Kidney Int 2011, 79, 624–634.
[16]
Zhou, T.B.; Qin, Y.H.; Lei, F.Y.; Su, L.N.; Zhao, Y.J.; Huang, W.F. All-trans retinoic acid regulates the expression of apolipoprotein E in rats with glomerulosclerosis induced by Adriamycin. Exp. Mol. Pathol 2011, 90, 287–294.
Zhang, D.; Sun, L.; Xian, W.; Liu, F.; Ling, G.; Xiao, L.; Liu, Y.; Peng, Y.; Haruna, Y.; Kanwar, Y.S. Low-dose paclitaxel ameliorates renal fibrosis in rat UUO model by inhibition of TGF-β/Smad activity. Lab. Invest 2010, 90, 436–447.
[29]
Radford, M.G., Jr; Donadio, J.V., Jr; Bergstralh, E.J.; Grande, J.P. Predicting renal outcome in IgA nephropathy. J. Am. Soc. Nephrol. 1997, 8, 199–207.
[30]
Zhou, T.B.; Qin, Y.H.; Zhou, C.; Lei, F.Y.; Zhao, Y.J.; Chen, J.; Su, L.N.; Huang, W.F. Less expression of prohibitin is associated with increased Caspase-3 expression and cell apoptosis in renal interstitial fibrosis rats. Nephrology (Carlton) 2012, 17, 189–196.
[31]
Hu, X.; Zhou, X.; He, B.; Xu, C.; Wu, L.; Cui, B.; Wen, H.; Lu, Z.; Jiang, H. Minocycline protects against myocardial ischemia and reperfusion injury by inhibiting high mobility group box 1 protein in rats. Eur. J. Pharmacol 2010, 638, 84–89.
[32]
Ha, T.; Hua, F.; Liu, X.; Ma, J.; McMullen, J.R.; Shioi, T.; Izumo, S.; Kelley, J.; Gao, X.; Browder, W.; et al. Lipopolysaccharide-induced myocardial protection against ischaemia/ reperfusion injury is mediated through a PI3K/Akt-dependent mechanism. Cardiovasc. Res 2008, 78, 546–553.
[33]
Li, W.; Wang, J.; Jiang, H.R.; Xu, X.L.; Zhang, J.; Liu, M.L.; Zhai, L.Y. Combined effects of cyclooxygenase-1 and cyclooxygenase-2 selective inhibitors on ovarian carcinoma in vivo. Int. J. Mol. Sci 2011, 12, 668–681.
[34]
Lai, J.Y.; Li, Y.T.; Wang, T.P. In vitro response of retinal pigment epithelial cells exposed to chitosan materials prepared with different cross-linkers. Int. J. Mol. Sci 2010, 11, 5256–5272.
[35]
Olalekan, L.A.; Lawal, A.F.; Ologundudu, A.; Adeniran, O.Y.; Omonkhua, A.; Obi, F. Antioxidant effects of heated garlic juice on cadmium-induced liver damage in rats as compared to ascorbic acid. J. Toxicol. Sci 2011, 36, 549–557.
[36]
Zhang, Y.; Peng, T.; Zhu, H.; Zheng, X.; Zhang, X.; Jiang, N.; Cheng, X.; Lai, X.; Shunnar, A.; Singh, M.; et al. Prevention of hyperglycemia-induced myocardial apoptosis by gene silencing of Toll-like receptor-4. J. Transl. Med 2010, 8, doi:10.1186/1479-5876-8-133.
[37]
Chen, N.; Ko, M. Schisandrin B-induced glutathione antioxidant response and cardioprotection are mediated by reactive oxidant species production in rat hearts. Biol. Pharm. Bull 2010, 33, 825–829.
[38]
Zhou, T.B.; Qin, Y.H.; Lei, F.Y.; Su, L.N.; Zhao, Y.J.; Huang, W.F. Less gelatinases is associated with apolipoprotein E accumulation in glomerulosclerosis rats. Histol. Histopathol 2012, 27, 249–256.
[39]
Zhou, T.B.; Qin, Y.H.; Lei, F.Y.; Zhao, Y.J.; Huang, W.F. Association of PAX2 with cell apoptosis in unilateral ureteral obstruction rats. Ren. Fail 2012, 34, 194–202.
