Cloning of SRY Gene from Moschus berezovskii and M.chrysogaster and Its Application in Phylogenetic Analysis
林麝及马麝SRY基因片段克隆及其在系统进化分析中的应用

A pair of degenerate primers was designed according to the reported SRY sequences about human, dog and Artiodactyla animals.Two 684 bp CDS regions of SRY gene were cloned and sequenced from male Moschus berezovskii and male M.chrysogaster genomic DNA by PCR method.Sequence analysis were conducted in the light of paternal inheritance and the SRY sequences from M.berezovskii,M.chrysogaster,and the other 18 Artiodactyla animals were used for phylogenetic tree construction by using neighbor-joining (NJ) analysis of MEGA2.1 and maximum parsimony method (MP) analysis of PAUP4.0.The phylogeny showed that the musk deer formed a monophyletic group and it supported that the musk deer should be classified as an individual family Moschidae,instead of a subfamily or a genus.