RAPD Analysis on Guizhou Native Goat Breeds
贵州地方山羊品种的RAPD分析

We used 180 primers in RAPD analysis of Guizhou native goats (the small xiang goat,Guizhou white goat,Guizhou black goat and Qianbei pockmarked goat),Nanjiang brown goats and Boer goats.Of the primers,27 amplified polymorphic patterns,including 281 bands.Of the bands,115 were polymorphic and their frequency was 40 92% on average (range 20%-80%).Each of the 27 primers had 10 41 bands on average (range 4-16),and the fragment length of products was 210-2?800?bp.Genetic distance index appeared the lowest between the Guizhou black goat and Guizhou white goat (0 0605),and the highest between the Boer goat and the other goats (0 1059-0 1488).NJ tree indicated that the Guizhou white goat was closely related to the Guizhou black goat,and the next was the Qianbei pockmarked goat;the small xiang goat was farther from the other Guizhou native goats than Nanjiang brown goat from.The results analysis suggest that the small xiang goat is a independent breed on genetics,and Guizhou native goat breeds have the close genetic relationship,low genetic variability and high genetic stability.