Sperm Cryopreservation in Sparus macrocephalus and DNA Damage Detection with SCGE
黑鲷精子的超低温冻存及DNA损伤的SCGE检测

In this paper, DMSO was used as cryoprotectant for cryopreservation of Sparus macrocephalus spermatozoa in 0.5 mL straws. Detection of DNA damage in response to a cryopreservation process in Sparus macrocephalus spermatozoa was also carried out. The results demonstrated that there were no significant differences between frozen-thawed sperm conserved by Cortland solution diluted with 5%, 10%, 15%, 20% DMSO and fresh sperm in motility. The best motility of frozen-thawed sperm were obtained when DMSO concentra...