CYTOPATHIC ACTION OF SPIDER (Lycosa singoriensis) VENOM AND ITS FRACTIONS ON THE CULTURED HUMAN LUNG ADENOCARCINOMA CELL(SPC-A1 )
穴居狼蛛毒的某些组分对培养的人肺腺癌细胞(SPC-A_1)的致伤作用

The spider crude venom(SV)was extracted from lyophilized poison glands of Lycosa singoriensis and was fractionated by means of chromatography on Sephadex G-25 into 8 fractions. It was shown that the minimal dosa of SV to kill 50% (MD50) of the cultured human lung adenocarcinoma cells (SPC-A1) was 25 ug/ml, whereas even 600 ug/ml of SV killed only 30% of the cultured human embryonic lung cells. Human lymphocytes were nearly as resistant as the embryonie lung cells and MD50 of SV on human erythrocyles wan more than 4000 ug/ml. The cytopathie activity on the adenocarcinoma cells was found in fractions III, IV and VIII. Moreover, the cytopathic activity of fraction IV and VIII was found to be heat resistant (100C, 30 min), but that of fraction I was disappeared after the heat treatment.