Complete Mitochondrial Genome and Secondary Structures of lrRNA and srRNA of Atractomorpha sinensis (Orthoptera, Pyrgomorphidae)
短额负蝗线粒体基因组及其lrRNA和srRNA二级结构分析

The complete sequence of Atractomorpha sinensis mitochondrial genome was determined using long PCR and conserved primer walking approaches. The results showed that the entire mitochondrial genome of A. sinensis is 15 558 bp long with A T content 74.3%. All 37 genes are conserved in the position observed in that of Locusta migratoria. Genes are closely assembled one after the other, leaving a total of 64 bp (excluding the A T rich region) in intergenic spacers, ranging in size from 1 bp to 16 bp. The extremities of some genes overlap with each other by a few nucleotides, of which 15 overlaps range in size from 1 bp to 8 bp, giving a total of 51 overlapping nucleotides. In the 13 protein coding genes (PCGs), six types of start codons were identified, in which TAA and TAG were used in 12 PCGs as stop codons, except ND5 (TA). Most tRNA genes could form typical secondary structures except tRNASer (AGN), which had an absence of the DHU arm. There are six domains, 49 helices and three domains, 33 helices in the secondary structures of lrRNA and srRNA, respectively. In addition, there is an A T rich region with 778 bp in length containing a polythymidine stretch that may be involved in replication and/or translation initiation.