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动物学研究 1995
THE ESTABLISHMENT AND PRESERVATION OF LYMPHOCYTE STRAIN OF SOME RARE PRIMATES
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Abstract:
This is a report of the comparative study of the chromosomes in B95-8 cell transformed by EBV with that of the normal Sagninus oedipus studied by Hsu T. C. For the normal, the diploid chromosomes number is 2n= 46 and karyotype consists of three groups: A, 2 pairs submetacentric and 2 pairs of micrometacentric; B, 11 pairs of submetacentric; C, 7 pairs of telocentric. One large metacentric X-chromosome and a microtelocentric Y-chromosome can be differentiated easily.For the B95-8 cell which has been transformed through carrying EBV 2n = 47, the A and C group of the karyotype appeared as the same as the normal Sagninus oeaipus. But for the B group, there are 3 chromosomes without pairing (one large metacentric; one large submetacentric and one microtelocentric) with the exception of No.10 submetacentric. However, the lymphocyte of Rhinopitheus bieti, Rhimopitheus rotellanae, Hylobates hoolock, Presbytis francoisi, Presbytls phayrei crepusculus as well as Presbytis nemaeus transformed by EBV the diploid chromosome number, G-, C-banding and Ag-NORs are all consistent with that of their normal ones.