|
动物学研究 1985
STUDIES ON ESTERASE ISOENZYMES IN ATTACUS CYNTHIA RICINI
|
Abstract:
Esterase isozyme comparison of different organs (the gut, integument fat body silk gland, hemolymph)of Attacus cynthia ricini were undertaken using the techniques of polyacrylamide gel electrophoresis. The larvae (the 5th day of the 5th instar) of Attacus cynthia ricini and black Bombyx mori (donor) and pure white Attacus cynthia ricini (recipient) and "Spotted worm" and "Spotted white worm" of Attacus cynthia ricini (transfer) were analyzed for Esterase isozyme. Our results indicated that zymogram for Esterase isozyme activity of different organs in all subspecies Attacus cynthia ricini showed clear differences in band number, their intensities and relative mobolities. The main esterase isozymic patterns of the same organ of Attacus cynthia ricini were consistent, but the zymograms for Esterase isozyme activityshowed different intensities, Their fine esterase isozymic band numbers differ slightly. The esterase isozymic patterns of the silk gland were indentical. Among the donor and recipient and trasfer the esterase isozymic patterns of different organ did not show the characteristic isozymic band Attacus cynthia ricini induced by Bombox mori DNA.