A NEW FLUORESCENT PROGESTERONE CONJUGATE FOR DETECTION OF PROGESTERONE RECEPTORS IN THE CHICK OVIDUCT
一种新的荧光孕酮探测鸡输卵管孕酮受体

6s (a-amino propioni acid)-fluorescein isothiocyanatc-progcsterone conjugate (6NHPF) was prepared by the method of chemical synthesis and it was used for detection of progesterone receptors (PR) in the chick oviduct. The results have shown that PR exist in the nuclei of luminal epithelium but neither in the smooth muscle and nor in the excretive and reproductive cavity. Furthmore, taking progesterone or intermediate compound of fluorescent progesterone conjugate as PR competitors, the fluorescent progesterone conjugate was effectively competed in the neighbouring sections. Therefore, this new, hydrophious, small moleculcr fluorescent progesterone conjugate was proved specifically combined with progesterone receptors.