A Gram-positive, facultatively anaerobic, endospore-forming and rod-shaped bacterium was isolated from soil samples and designated strain LQQ. This organism strongly quenches the acylhomoserine lactone quorum-sensing signal. The LQQ strain exhibits phenotypic characteristics consistent with its classification in the genus Bacillus. It is positive in catalase and no special growth factor is needed. It uses glucose as sole carbon source. The DNA G + C content is 39.8 mol %. The closest relatives based on the 16S rRNA gene sequence are Bacillus anthracis, Bacillus thuringiensis, and Brevibacillus brevis (syn. Bacillus brevis) with the similarity of 96.5%. The DNA–DNA hybridization data indicates a low level of genomic relatedness with the relative type strains of Bacillus thuringiensis (6.1%), Bacillus anthracis (10.5%) and Brevibacillus brevis (8.7%). On the basis of the phenotypic and phylogenetic data together with the genomic distinctiveness, the LQQ strain represents a novel species of the genus Bacillus, for which the name Bacillus marcorestinctum sp. nov. is proposed. The type strain is LQQ T.
References
[1]
Reading, NC; Sperandio, V. Quorum sensing: The many languages of bacteria. FEMS Microbiol. Lett？2006, 254, 1–11, doi:10.1111/j.1574-6968.2005.00001.x. 16451172
[2]
Roche, DM; Byers, JT; Smith, DS; Glansdorp, FG; Spring, DR; Welch, M. Communications blackout? Do N-acylhomoserinelactone-degrading enzymes have any role in quorum sensing? Microbiology？2004, 150, 2023–2028, doi:10.1099/mic.0.26977-0. 15256546
[3]
Hentzer, M; Givskov, M. Pharmacological inhibition of quorum sensing for the treatment of chronic bacterial infections. J. Clin. Invest？2003, 112, 1300–1307. 14597754
[4]
Medina-Martínez, MS; Uyttendaele, M; Rajkovic, A; Nadal, P; Debevere, J. Degradation of N-acyl-L-homoserine lactones by Bacillus cereus in culture media and pork extract. Appl. Environ. Microbiol？2007, 73, 2329–2332, doi:10.1128/AEM.01993-06. 17293532
[5]
Dong, YH; Zhang, LH. Quorum sensing and quorum-quenching enzymes. J. Microbiol？2005, 143, 101–109.
[6]
Huang, JJ; Petersen, A; Whiteley, M; Leadbetter, JR. Identification of QuiP, the product of gene PA1032, as the second acyl-homoserinelactone acylase of Pseudomonas aeruginosa PAO1. Appl. Environ. Microbiol？2006, 72, 1190–1197, doi:10.1128/AEM.72.2.1190-1197.2006. 16461666
[7]
Sio, CF; Otten, LG; Cool, RH; Diggle, SP; Braun, PG; Bos, R; Daykin, M; Cámara, M; Williams, P; Quax, WJ. Quorum quenching by an N-acyl-homoserine lactone acylase from Pseudomonas aeruginosa PAO1. Infect. Immunity？2006, 74, 1673–1682, doi:10.1128/IAI.74.3.1673-1682.2006.
[8]
Dong, YH; Xu, JL; Li, X; Zhang, LH. AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora. Proc. Natl. Acad. Sci. USA？2000, 97, 3526–3531, doi:10.1073/pnas.97.7.3526. 10716724
[9]
Lin, YH; Xu, JL; Hu, J; Wang, LH; Ong, SL; Leadbetter, JR; Zhang, LH. Acyl-homoserine lactone acylase from Ralstonia strain XJ12B represents a novel and potent class of quorum-quenching enzymes. Mol. Microbiol？2003, 47, 849–860, doi:10.1046/j.1365-2958.2003.03351.x. 12535081
[10]
Uroz, S; Chhabra, SR; Cámara, N; Williams, P; Oger, P; Dessaux, Y. N-Acylhomoserine lactone quorum-sensing molecules are modified and degraded by Rhodococcus erythropolis W2 by both amidolytic and novel oxidoreductase activities. Microbiology？2005, 151, 3313–3322, doi:10.1099/mic.0.27961-0. 16207914
[11]
Zhang, HB; Wang, LH; Zhang, LH. Genetic control of quorum-sensing signal turnover in Agrobacterium tumefaciens. Proc. Natl. Acad. Sci. USA？2002, 99, 4638–4643, doi:10.1073/pnas.022056699. 11930013
[12]
Uroz, S; D’Angelo-Picard, C; Carlier, A; Elasri, M; Sicot, C; Petit, A; Oger, P; Faure, D; Dessaux, Y. Novel bacteria degrading N-acylhomoserine lactones and their use as quenchers of quorum-sensing-regulated functions of plant-pathogenic bacteria. Microbiology？2003, 149, 1981–1989, doi:10.1099/mic.0.26375-0. 12904538
[13]
Leadbetter, JR; Greenberg, EP. Metabolism of acylhomoserine lactone quorum-sensing signals by Variovorax paradoxus. J. Bacteriol？2000, 182, 6921–6926, doi:10.1128/JB.182.24.6921-6926.2000. 11092851
[14]
Johnson, M; Zaretskaya, I; Raytselis, Y; Merezhuk, Y; McGinnis, S; Madden, TL. NCBI BLAST: a better web interface. Nucl. Acids Res？2008, 36, W5–W9, doi:10.1093/nar/gkn201. 18440982
[15]
Cole, JR; Wang, Q; Cardenas, E; Fish, J; Chai, B; Farris, RJ; Kulam-Syed-Mohideen, AS; McGarrell, DM; Marsh, T; Garrity, GM; Tiedje, JM. The Ribosomal Database Project: improved alignments and new tools for rRNA analysis. Nucl. Acids Res？2009, 37, D141–D145, doi:10.1093/nar/gkn879. 19004872
[16]
Claus, D; Berkeley, RCW. Genus Bacillus Cohn 1872,174. In Bergey’s Manual of Systematic Bacteriology; Sneath, PHA, Mair, NS, Sharpe, ME, Holt, JG, Eds.; Williams & Wilkins: London, UK, 1994; Volume 2, pp. 105–1139.
[17]
Wagner-D？bler, I; Rheims, H; Felske, A; El-Ghezal, A; Flade-Schr？der, D; Laatsch, H; Lang, S; Pukall, R; Tindall, BJ. Oceanibulbus indolifex, gen. nov., sp. nov., a North Sea Alphaproteo-bacterium producing bioactive metabolites. Int. J. Syst. Evol. Microbiol？2004, 54, 1177–1184, doi:10.1099/ijs.0.02850-0. 15280288
[18]
Wayne, LG; Brenner, DJ; Colwell, RR; Grimont, PAD; Kandler, O; Krichevsky, MI; Moore, LH; Moore, WEC; Murray, RGE; Stackebrandt, E; Starr, MP; Truper, HG. International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int. J. Syst. Bacteriol？1987, 37, 463–464, doi:10.1099/00207713-37-4-463.
Piper, K; Beck, R; von Bodman, S; Farrand, SK. Conjugation factor of Agrobacterium tumefaciens regulates Ti plasmid transfer by autoinduction. Nature (London)？1993, 362, 448–450, doi:10.1038/362448a0.
[21]
Dong, W; Luo, F; Du, Y; Bai, X; Li, X. Production and properties of an inhibitor of the Pseudomonas autoinducer by Pseudomonas aeruginosa. Can. J. Microbiol？2005, 51, 783–789, doi:10.1139/w05-066. 16391658
[22]
Costa, JM; Loper, JE. EcbI and EcbR: Homologs of LuxI and LuxR affecting antibiotic and exoenzyme production by Erwinia carotovora subsp betavasculorum. Can. J. Microbiol？1997, 43, 1164–1171, doi:10.1139/m97-165. 9476353
[23]
Murray, RGE; Doetsch, RN; Robinow, CF. Determinative and cytological light microscopy. In Methods for General and Molecular Bacteriology; Gerhardt, P, Murray, RGE, Wood, WA, Krieg, NR, Eds.; American Society for Microbiology: Washington D.C., USA, 1994; pp. 21–41.
[24]
Smibert, RM; Krieg, NR. Phenotypic characterization. In Methods for General and Molecular Bacteriology; Gerhardt, P, Murray, RGE, Wood, WA, Krieg, NR, Eds.; American Society for Microbiology: Washington D.C., USA, 1994; pp. 607–654.
[25]
Moore, DD; Dowhan, D. Preparation and analysis of DNA. In Current Protocols in Molecular Biology; Ausubel, FM, Brent, R, Kingston, RE, Moore, DD, Seidman, JG, Smith, JA, Struhl, R, Eds.; John Wiley & Sons: New York, NY, USA, 1997; Volume 1, pp. 241–245.
[26]
Marmur, J; Doty, P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. Mol. Biol？1962, 5, 109–118, doi:10.1016/S0022-2836(62)80066-7. 14470099
[27]
Ziemke, F; Hofle, MG; Lalucat, J; Rossello-Mora, R. Reclassification of Shewanella putrefaciens owen’s genomic group II as Shewanella baltica sp. nov. Int. J. Syst. Bacteriol？1998, 48, 179–186, doi:10.1099/00207713-48-1-179. 9542087
[28]
Li, X; Zhang, D; Chen, F; Ma, J; Dong, Y; Zhang, L. Klebsiella singaporensis sp. nov., a novel isomaltulose-producing bacterium. Int. J. Syst. Evol. Microbiol？2004, 54, 2131–2136, doi:10.1099/ijs.0.02690-0. 15545446
[29]
Thompson, JD; Gibson, TJ; Plewniak, F; Jeanmougin, F; Higgins, DG. The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl. Acids Res？1997, 24, 4876–4882.
[30]
Felsenstein, J. PHYLIP (Phylogeny Inference Package) version 36; Department of Genome Sciences, University of Washington: Seattle, WA, USA, 2005.
