In this study, the knowledge, attitudes and practices regarding pesticide use and the levels of exposure of farmers and residents to organophosphorous and/or carbamates pesticides were evaluated in two rural settings in Brazil. A questionnaire was completed by 112 farm workers aged ≥18 years. Almost all farmers acknowledged that pesticides were potentially harmful to their health (87.5%); however, over half rarely (48.2%) or never (7.2%) used personal protective devices (PPDs). An association was found ( p = 0.001) between the work regimen and the use of PPDs, with more frequent equipment use among hired laborers than those involved in family agriculture. A significant correlation ( p = 0.027) was found between the reporting of adverse symptoms and the use of backpack sprayers. Mean AChE activities of farmers (n = 64) and residents (n = 18) during the exposure and non-exposure periods were significantly lower than their control groups. Mean BChE activities of farmers and residents were significantly lower than their controls during the exposure period. Among the 60 farmers that had blood samples collected in both the exposure and non-exposure (baseline) periods, 10 (16.7%) had AChE depletion of over 30% during the exposure period compared with the baseline level. Six residents living on the same farms also presented this depletion. AChE was over 30% higher than the baseline level for 19 farmers (31.7%), indicating a reboot effect. Special education programs are needed in these regions to promote the safe use of pesticides in the field to decrease the risks from exposure to pesticides for farmers, and from secondary exposure to these compounds for their families.
Sam, K.G.; Andrade, H.H.; Pradhan, L.; Pradhan, A.; Sones, S.J.; Rao, P.G.M.; Sudhakar, C. Effectiveness of an education program to promote pesticides safety among pesticides handlers of South índia. Int. Arch. Occup. Environ. Health 2008, 81, 787–795, doi:10.1007/s00420-007-0263-3.
[3]
Faria, N.M.X.; Rosa, J.A.R.; Facchini, L.A. Poisoning by pesticides among family fruit farmers, Bento Gon？alves, Southern Brazil. Rev. Saude Publ. 2009, 43, 335–344, doi:10.1590/S0034-89102009005000014.
[4]
Rebelo, R.M.; Vasconcelos, R.A.; Buys, B.D.M.C.; Rezende, J.A.; Moraes, K.O.C.; Oliveira, R.P. Pesticides and Related Commercialized in Brazil in 2009. An Environmental Approach; IBAMA Press: Brasilia, Brazil, 2010; pp. 30–84.
[5]
Brazilian Institute of Geography and Statistics. Censo Agropecuário, 2006. Available online: http://www.sidra.ibge.gov.br/bda/pesquisas/ca/default.asp?o=2&i=P (accessed on 13 August 2012).
[6]
Brazilian Ministry of Agriculture, Livestock and Food Supply. Agrofit, Sistema de Agrotóxicos Fitossanitário, 2012. Available online: http://extranet.agricultura.gov.br/agrofit_cons/principal_agrofit_cons (accessed on 13 August 2012).
[7]
Brazilian System of Toxico-Pharmacological Information, 2012. Available online: http://www.fiocruz.br/sinitox (accessed on 13 August 2012).
[8]
International Programme on Chemical Safety. Guidelines on the Prevention of Toxic Exposures. Education and Public Awareness Activities; World Health Organization: Geneva, Switzerland, 2004. Available online: http://www.Who.Int/Ipcs/Features/Prevention_Guidelines.Pdf (accessed on 13 August 2012).
[9]
Blanco, L.E.; Aragón, A.; Lundberg, I.; Lidén, C.; Wesseling, C.; Nise, G. Determinants of dermal exposure among Nicaraguan subsistence farmers during pesticide applications with backpack sprayers. Ann. Occup. Hyg. 2005, 49, 17–24.
[10]
Ngowi, A.V.; Maeda, D.N.; Partanen, T.J. Knowledge, attitudes and practices (KAP) among agricultural extension workers concerning the reduction of the adverse impact of pesticides in agricultural areas in Tanzania. Med. Lav. 2002, 93, 338–346.
[11]
Recena, M.C.P.; Caldas, E.D. Risk perception, attitudes and practices on pesticide use among farmers of a city in Midwestern Brazil. Rev. Saude Publ. 2008, 42, 294–301.
[12]
Ospina, J.M.; Manrique-Abril, F.G.; Ariza, N.E. Educational intervention concerning knowledge and practices regarding work-related risks in potato farmers in Boyacá, Colombia. Rev. Salud Publ. 2009, 11, 182–190, doi:10.1590/S0124-00642009000200003.
[13]
Karunamoorthi, K.; Mohammed, A.; Jemal, Z. Peasant association member’s knowledge, attitudes, and practices towards safe use of pesticide management. Am. J. Ind. Med. 2011, 54, 965–970, doi:10.1002/ajim.21008.
[14]
Ribeiro, M.G.; Colasso, C.G.; Monteiro, P.P.; Filho, W.R.; Yonamine, M. Occupational safety and health practices among flower greenhouses workers from Alto Tietê region (Brazil). Sci. Total Environ. 2012, 416, 121–126, doi:10.1016/j.scitotenv.2011.11.002.
[15]
Konradsen, F.; van der Hoek, W.; Cole, D.C.; Hutchinson, G.; Daisley, H.; Singh, S.; Eddleston, M. Reducing acute poisoning in developing countries-Options for restricting the availability of pesticides. Toxicology 2003, 192, 249–261, doi:10.1016/S0300-483X(03)00339-1.
[16]
United States Environmental Protection Agency. Agreement to Terminate All Uses of Aldicarb, 2010. Available online: http://www.epa.gov/oppsrrd1/REDs/factsheets/aldicarb_fs.html (accessed on 13 August 2012).
[17]
Brazilian Health Surveilance Agency. Agrotóxicos e Toxicologia. Legisla？？o, 2011. Available online: http://www.anvisa.gov.br (accessed on 13 August 2012).
[18]
Ecobichon, D.J. Toxic Effects of Testicides. In Casarett and Doull’s Toxicology: The Basic Science of Poisons; McGraw-Hill: New York, NY, USA, 2001; pp. 673–709.
[19]
Lotti, M. Clinical Toxicology of Anticholinesterase Agents in Humans. In Handbook of Pesticides Toxicology: Principles; Krieger, R.I., Krieger, W.C., Eds.; Elsevier Academic Press: San Diego, CA, USA, 2001; pp. 1043–1085.
[20]
Colosio, C.; Tiramani, M.; Maroni, M. Neurobehavioral effects of pesticides: State of the art. NeuroToxicology 2003, 24, 577–591, doi:10.1016/S0161-813X(03)00055-X.
[21]
Stefanidou, M.; Athanaselis, S.; Spiliopoulou, H. Butirylcholinesterase: Biomarker for exposure to organophosphorus insecticides. Intern. Med. J. 2009, 29, 57–60.
[22]
Jintana, S.; Sming, K.; Krongtong, Y.; Thanyachai, S. Cholinesterase activity, pesticide exposure and health impact in a population exposed to organophosphate. Int. Arch. Occup. Environ. Health 2009, 82, 833–842, doi:10.1007/s00420-009-0422-9.
[23]
Mason, H.J.; Sams, C.; Stevenson, A.J.; Rawbone, R. Rates of spontaneous reactivation and aging of acetylcholinesterase in human erythrocytes after inhibition by organophosphorus pesticides. Hum. Exp. Toxicol. 2000, 19, 511–516, doi:10.1191/096032700667340089.
[24]
Cocker, J.; Mason, H.J.; Garfitt, S.J.; Jones, K. Biological monitoring of exposure to organophosphate pesticides. Toxicol. Lett. 2002, 134, 97–103, doi:10.1016/S0378-4274(02)00168-6.
[25]
Santos, M.M.; Noronha, J.F. Diagnóstico da cultura do tomate de mesa no município de Goianápolis, estado de Goiás, Brasil. Pesq. Agrop. Trop. 2001, 31, 29–34.
Ellman, G.L.; Courtney, K.D.; Andres, V., Jr.; Feather-Stone, R.M. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol. 1961, 7, 88–95.
[28]
Oliveira-Silva, J.J.; Alves, S.R.; Inácio, A.F.; Meyer, A.; Sarcinelli, P.N.; Mattos, R.C.; Ferreira, M.F.A.; Cunha, J.C.; Moreira, J.C. Cholinesterase activities determination in frozen blood samples: An improvement to theoccupational monitoring in developing countries. Hum. Exp. Toxicol. 2000, 19, 173–177, doi:10.1191/096032700678827762.
[29]
Presidência da República Casa Civil Subchefia para Assuntos Jurídicos. 1989. Available online: http://www.planalto.gov.br/ccivil_03/leis/l7802.htm (accessed on 13 August 2012).
[30]
Waichman, A.V.; Eveb, E.; Nina, N.C.S. Do farmers understand the information displayed on pesticide product labels? A key question to reduce pesticides exposure and risk of poisoning in the Brazilian Amazon. Crop. Prot. 2007, 26, 576–583, doi:10.1016/j.cropro.2006.05.011.
[31]
Zhang, X.; Zhao, W.; Jing, R.; Wheeler, K.; Smith, G.A.; Stallones, L.; Xiang, H. Work-related pesticide poisoning among farmers in two villages of Southern China: A cross-sectional survey. BMC Public Health 2011, 11, 429–436, doi:10.1186/1471-2458-11-429.
[32]
Moreira, J.C.; Jacob, S.C.; Peres, F.; Lima, J.S.; Meyer, A.; Oliveira-Silva, J.J.; Sarcinelli, P.N.; Batista, D.F.; Egler, M.; Faria, M.V.C.; et al. Integrated evaluation of the health impact of pesticide use in a community at Nova Friburgo, RJ. Ciênc Saúde Col. 2002, 7, 299–311.
[33]
Damalas, C.A.; Georgiou, E.B.; Theodorou, M.G. Pesticide use and safety practices among Greek tobacco farmers: A survey. Int. J. Environ. Health Res. 2006, 16, 339–348, doi:10.1080/09603120600869190.
[34]
Stokes, L.; Stark, A.; Marshall, E.; Narang, A. Neurotoxicity among pesticide applicators exposed to organophosphates. Occup. Environ. Med. 1995, 52, 648–653, doi:10.1136/oem.52.10.648.
[35]
Instituto Nacional de Processamento de Embalagens vazias (INPEV). 2011. Available online: http://www.inpev.org.br (accessed on 13 August 2012).
[36]
Bigat？o, D.A.R. Cuidados e destina？？o final de embalagens, na utiliza？？o de agrotóxicos por produtores rurais no município de Itapor？. M.S. Thesis, University of Brasília, Federal District, Brazil, 2009.
[37]
Silva, J.M.; Novato-Silva, E.; Faria, H.P.; Pinheiro, T.M.M. Pesticides and work: A dangerous combination for the Brazilian agricultural worker’s health. Ciênc Saúde Col. 2005, 10, 891–903, doi:10.1590/S1413-81232005000400013.
[38]
Garcia, A.M.; Ramirez, A.; Lacasa？a, M. Prácticas de utilización de plaguicidas en agricultores. Gac. Sanit. 2002, 16, 236–240.
[39]
Soares, W.; Almeida, R.M.V.R.; Moro, S. Rural work and risk factors associated with pesticide use in Minas Gerais, Brazil. Cad. Saúde Públ. 2003, 19, 1117–1127, doi:10.1590/S0102-311X2003000400033.
[40]
Brazilian Ministry of Labor and Employment. Norma Regulamentadora 07, 1994. Available online: http://portal.mte.gov.br/data/files/8A7C812D308E21660130E0819FC102ED/nr_07.pdf (accessed on 13 August 2012).
[41]
International Programme on Food Safety. Organophousphorus Insecticides: A General Introduction; Environmental Health Criteria 63; World Health Organization: Geneva, Switzerland, 1986. Available online: http://www.inchem.org/documents/ehc/ehc/ehc63.htm (accessed on 13 August 2012).
[42]
Maroni, M.; Colosio, C.; Ferioli, A.; Fait, A. Organophosphorus pesticides. Toxicology 2000, 143, 9–37.
Reiner, E.; Simeon-Rudolf, V. Methods for Measuring Cholinesterase Activities in Human Blood. In Toxicology of Organophosphate & Carbamate Compounds; Gupta, R.C., Ed.; Elsevier Academic Press: San Diego, CA, USA, 2006; pp. 199–208.
[45]
Division of Occupational Safety & Health (DOSH). Cholinesterase Monitoring for Agricultural Pesticides Handlers. Department of Labor & Industries, 2010. Available online: http://www.lni.wa.gov/Safety/Topics/AtoZ/Cholinesterase/files/ProvidersGuidelines1.pdf (accessed on 13 August 2012).
[46]
Peres, F.; Oliveira-Silva, J.J.; Della-Rosa, H.V.; de Lucca, S.R. Challenges in the study of human and environmental contamination by pesticides. Cienc Saude Col. 2005, 10(Suppl), 27–37.
[47]
Gallo, M.A.; Lawryk, N.J. Organic Phosphorous Pesticides. In Handbook of Pesticide Toxicology, 2nd; Hayes, W.J., Laws, E.R., Eds.; Elsevier Academic Press: San Diego, CA, USA, 1991; pp. 917–1123.
[48]
Eskenazi, B.; Harley, K.; Bradman, A.; Weltzien, E.; Jewell, N.P.; Barr, D.B.; Furlong, C.E.; Holland, N.T. Association of in utero organophosphate pesticide exposure and fetal growth and length of gestation in an agricultural population. Environ. Health Perspect. 2004, 112, 1116–1124, doi:10.1289/ehp.6789.
[49]
Silva de Assis, H.C.; Nicaretta, L.; Marques, M.C.A.M.; Crestani, S.; Soares, K.C.; Olmedo, A.; Dalsenter, P.R. Anticholinesterasic activity of endosulfan in wistar rats. Bull. Environ. Contam. Toxicol. 2011, 86, 368–372.
[50]
Hedt, B.L.; Pagano, M. Health indicators: Eliminating bias from convenience sampling estimators. Stat. Med. 2011, 30, 560–568.
