Gene Expression Analysis of CD34 + Hematopoietic Stem and Progenitor Cells Grown in Different Culture Environments Using Differential Display
用差异显示法分析不同生长环境中的造血干细胞/祖细胞基因表达的初步研究

Objective: To investigate the changes of gene expression in CD34 hematopoietic stem and progenitor cells (HSPCs) under different growth environments. Methods: Umbilical cord blood mononuclear cells (UCB MNCs) were cultured in static and stirred systems. After 7 days of culture, CD34 cells were isolated and total RNA was extracted. Gene expression patterns of CD34 cells from fresh, static and stirred cultures were compared using differential display (DD). Results: 30 gene fragments displayed differential expression levels based on the conditions of DD. One of differentially expressed genes was identified as RAN, which is a member of oncogene RAS family. This gene may be associated with proliferation of hematopoietic cells. Conclusion: Different growth environments induced differential gene expression patterns of CD34 HSPCs. These differentially expressed genes would give new insights into optimizing in vitro environments for expanding hematopoietic cells.