Localization of Arabidopsis VHA-c3 Promoter with GUS-Fusion
拟南芥VHA-c3启动子的GUS基因融合表达

VHA-c3 gene is one of the five homologous genes which belong to the subunits of vacuole H+-ATPase in Arabidopsis thaliana. Former reports indicated that it is related with resistance to abiotic stress in plants. In this article, different fragments of the upstream regulation sequences of Arabidopsis VHA-c3 gene were cloned, and fused with GUS reporter gene, to study the localization of VHA-c3 gene. The results showed that: The essential element for the basal activity of VHA-c3 is located within the -722bp fragments upstream of the translation start codon of VHA-c3, it can guide genes' constitutive expression in leaf blade, trichome, petiole, root, stamen, stigma and sepal in Arabidopsis; And there is two negative regulatory elements located in -2812bp to -2234bp and -1496bp to -772bp respectively, and an up-regulating cis-element from -2234bp to -1496bp fragment of VHA-c3 gene, which may control the gene's expression in guard cell.