Effect of flow cytometrically-sorted sperm on chromosomes of bovine blastocysts produced in vitro
牛分离精子对其IVF胚胎染色体影响的研究

The chromosome abnormalities of blastocysts (6 d ~ 8 d ) produced by IVF(in vitro fertilization) with flow cytometrically-sorted, stained and unsorted sperm from 3 bulls were investigate in this study, which determine if the sorting procedure and staining cause the chromosomal abnormality of embryos. The results revealed that there were no significant differences in chromosomal abnormality (mosaic) rates of blastocysts derived from sorted, stained unsorted and unsorted sperm (40.7%, 59/145; 35.8%, 40/106 and37.0%,37/100, respectively); Chromosomal abnormality rates were variant among bulls (30.0% vs 44.6%; p<0.05); These results indicated that: 1) neither sorting procedure nor staining affect the chromosomal composition of embryos; The chromosomal abnormality rates of embryos were variable among bulls.