Studies on expression of PGA gene in Saccharomyces cerevisiae
PGA基因在酿酒酵母中的表达研究

In this study, the PGA gene of Bacillus megaterium 1.1741 was amplified by PCR, using primers designed on the base of PGA gene sequences of Bacillus megaterium in GenBank. Then the amplified PGA gene was cloned into pYES2 (amp+ ura+) vector, with T7lac promoter as the control. The constructed recombinant plasmid, named as pYES2-PGA, was transformed into Saccharomyces cerevisiae H158 (his- trp- leu- ura-) according to the LiAc/SSDNA/PEG method. PGA activity was detected in the culture liquid of recombinant Saccharomyces cerevisiae without penylacetic acid as the inducer, and the highest PGA activity was 0.75 U/ml. The sequence of PGA gene (present study) showed a high homology of 97.1%, 99.8% and 99.8% with three other strains of Bacillus megaterium L04471.1, U07682.1 and Z37542 in GenBank, respectively.