Cloning the multi-gene of isoflavonoid biosynthetic pathway with the SMART strategy
利用SMART方法快速克隆异黄酮代谢途径多基因的研究

High quality of total RNA from soybean leaves suitable for high efficient construction of cDNA library and gene cloning based on SMART strategy was isolated by the improved Acidic-Phenolic method. Five full length genes of isoflavonoid biosynthetic pathway were successfully and quickly amplified by PCR method with the original library cDNA dilution as templates. Comparing with traditional method of cloning genes from DNA or RNA, or isolating genes from cDNA plasmid hosted bacteria, this method can obtain full-length muhi-gene more quickly and simply.